Intermediate

Part:BBa_K542012:Design

Designed by: Anthony Vuong   Group: iGEM11_Lethbridge   (2011-09-28)


Catechol 2,3-dioxygenase with a C-terminal Arg-tag and Double Terminator (no promoter/RBS)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 311
    Illegal NgoMIV site found at 483
    Illegal AgeI site found at 834
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Arginine tag intended to allow for targeting into Lumazine Synthase microcompartment (BBa_K249002).


Source

(BBa_K542011) was assembled by the Lethbridge 2011 team and (BBa_B0015) was obtained from iGEM Distribution plates.

References